Unlocking the power of single molecule counting in your lab.
With single molecule counting, life is so much easier.
Multiplex assays — simple.
Manual thresholding and Poisson correction — not needed.
Inconsistent results — gone.
In the single molecule realm, PCR behaves fundamentally different.
Whether rare or abundant, every target becomes a single-molecule template — amplified without competition or distortion. Countable PCR delivers clean, unbiased amplification without the need for NGS or UMIs. Just pure signal, counted directly.
Challenging templates? No problem. Each compartment amplifies independently, making even long or low-efficiency targets quantifiable. Successfully amplify up to 5 kb — unlocking regions traditional PCR can't reach.
With single-molecule isolation, targets no longer compete — so multiplexing becomes fast and frustration-free. Panels that once took weeks to balance now come together in days, with minimal optimization and maximum clarity.
Single molecule detection begins with the Countable Matrix.
At the heart of Countable PCR is a structured gel – the Countable Matrix – that contains ~30 million compartments.
With that many compartments, it's easy to physically isolate single molecules in 3D space, making detection and counting simple, direct, and precise.
To create the Countable Matrix, the PCR mix is spun through a miniaturized spin column that fits into a familiar PCR tube.
After centrifugation, each molecule is captured in its own compartment, locked in space, and ready to be counted — enabling true single-molecule resolution in a simple, scalable format.
Single molecules are directly counted – right inside a PCR tube.
Countable PCR uses light sheet imaging to visualize the Countable Matrix in 3D – imaging the entire tube so no information is lost.
In just 40 seconds, ~30 million compartments are scanned in 3D — capturing single-molecule data with speed and precision.
The result? Instant reconstruction and direct molecule counts, with no thresholds, no Poisson correction, and no ambiguity.