Capture single-molecule suspensions of your targets with technology that generates a 3D matrix around your target DNA.
A mix-and-go protocol creates the suspensions of your target so that only single pieces of DNA occupy each compartment. So many compartments are generated, there is no concern about multiple targets occupying a single compartment.
This suspension enables direct counting of individual amplicons in the same tube; it also reduces competition for reaction reagents.
With true single-molecule encapsulation, you get high-sensitivity, low-variability counts of your gene targets.
Advanced light-sheet imaging scans your whole tube in less than 40 seconds, directly imaging your amplification products. The software’s algorithm automatically counts the number of molecules in your prep.
With direct counting, immediately get the number of target amplicons you have – eliminate extra time and material spent setting up standard curves, and avoid dilutions and bioinformatics needed for statistical modeling.
Reduce assay optimization time, as the signal from single molecule encapsulation is clean, without the ambiguities of mixed molecule partitions observed in conventional dPCR.
The Universal Multiplexing Kit makes it easier than ever to develop assays that target multiple markers. The kit contains probes that specifically target your gene of interest using standard oligomeric primers.
Develop new assays faster and with less expense compared to when you use hydrolysis probes – forward and reverse primers for Universal Multiplexing are standard oligomers that are low-cost to purchase and have a short delivery time.
Capture single-molecule suspensions of your targets with technology that generates a rigid 3D matrix around your target DNA.
A mix-and-go protocol creates the rigid suspension of your target so that only single pieces of DNA are compartmentalized.
This suspension enables direct counting of individual amplicons in the same tube; it also reduces competition for reaction reagents.
With true single-molecule encapsulation, you get high-sensitivity, low-variability counts of your gene targets.
Advanced light-sheet imaging scans your whole tube in <40 seconds, directly imaging your amplification products. The software’s algorithm automatically counts the number of molecules in your prep.
With direct counting, immediately get the number of target amplicons you have – eliminate extra time and material spent setting up standard curves, and avoid dilutions and bioinformatics needed for statistical modeling.
Reduce assay optimization time, as the signal from single molecule encapsulation is clean, without the ambiguities of mixed molecule partitions observed in conventional dPCR.
The Universal Multiplexing Kit makes it easier than ever to develop assays that target multiple markers, using standard oligomeric primers.
Develop new assays faster and with less expense compared to when you use hydrolysis probes – forward and reverse primers for Universal Multiplexing are standard oligomers that are low-cost to purchase and have a short delivery time.
The technological innovations behind Countable PCR are simple to add to your workflows. Your sample is encapsulated into compartments, amplified, and analyzed all in the same tube – ensuring you capture every event, without losing sample to dead volume.
Add your DNA sample for analysis to a simple spin column with Countable Matrix Reagent
A 20-minute centrifuge step isolates single DNA molecules individual compartments for precise quantification
Use a standard thermocycler to amplify signal from single molecules. Multiplex assays work effortlessly with targets amplified in isolation.
In less than 5 minutes, directly image and analyze your sample. The result? True, absolute count without standard curved or Poisson correction.
Add your DNA sample for analysis to a simple spin column with Countable Matrix Reagent
A 20-minute centrifuge step isolates single DNA molecules into ~30 million individual compartments for precise quantification
Use a standard thermocycler to amplify signal from single molecules. Multiplex assays work effortlessly with targets amplified in isolation.
In less than 5 minutes, directly image and analyze your sample.
The result? True, absolute count without standard curves or Poisson correction.
Countable PCR beats the drawbacks and limitations of qPCR (quantitative PCR, or real time PCR/RT-PCR) and dPCR (digital PCR or digital droplet PCR/ddPCR).
No more estimations – Get actual counts of your gene targets, no more estimations with ct counts
Skip expensive TaqMan probes – Use widely available oligomeric primers with Universal Multiplex technology for multiple gene-of-interest analysis
Slash labor and material costs – Use Universal Multiplexing and replace 96 qPCR reactions with 8 Countable PCR reactions
Avoid difficult optimization for multiplexing – Measure up to four targets in a single tube, without creating multiple standard curves and control experiments
Detect molecules at LODs previously unachievable – Count molecules with high sensitivity and precision
No more statistical estimation – Get direct, actual counts of your gene targets, no more statistical errors from Poisson correction
No more assumptions about single occupancy partitions – Single-molecule compartments ensure only individual molecules are amplified and counted
Avoid time and money costs associated with hydrolysis probes – Skip expensive TaqMan probes and use widely available oligomeric primers with Universal Multiplex technology for the same gene-of-interest analysis
Eliminate guesswork from thresholding and dual-occupancy – Multiplex up to four targets at once, with straightforward data analysis
Capture ultra-rare events alongside common events in the same sample – Work across a broad dynamic range in the same prep, so you count frequent and rare events in the same tube, without dilutions
