New to Universal Multiplexing? Start with this blog before diving in.
If you have optimized PCR primers for SYBR Green or other dye-based singleplex assays, you’re closer to multiplexing than you thought.
With Countable PCR, the Universal Multiplexing (UM) kit, and the online UM Converter you will quickly go from singleplex to multiplex PCR.
The online UM Converter (find the alpha version in the Support Center) takes your existing SYBR assays and instantly adapts them for Countable PCR Universal Multiplexing — no redesign, no long optimization cycles.
All it takes is pasting in your existing primer sequences, a couple of clicks, and the Converter does the rest.
Why you’ll actually want to multiplex now
Multiplexing has always promised to save time and sample, but in reality, setting up multiplex assays often costs both.
Using hydrolysis probes calls for a balancing game of primer design, probe placement, fluorescent channel separation, and endless interference testing.
The result is weeks of trial-and-error and budget drain — and the reasons why you’re back to SYBR or other dye-based PCR assays.
The UM Converter skips the pain and bridges the gap between your current SYBR Green primers and multiplex-ready primers — no reoptimization required!
What happens behind the scenes
First, the UM Converter evaluates a few primer-related parameters that may have little impact in singleplex assays, but can be more affected in multiplexing assays:
- Primer-to-primer interactions
- Secondary structures that impede binding
- 3’ extendible ends that risk primer dimers
- Non-specific interactions between primers and amplicons
It then uses those parameters to adapt your primers with UM adapters and identifies the optimal UM probe for your target set.
You get a clean, multiplexable assay design output in under a minute.
Multiplexing assay design in a couple of clicks vs. weeks
Say you have three validated SYBR assays. Traditionally, moving them into a hydrolysis probe multiplex assay would take days or weeks of redesign and validation to ensure:
- Probes don’t interfere with your targets
- Probe concentrations are tuned for balanced signals
- Reaction conditions are optimized to minimize amplification bias
With the UM Converter, those same primers are adapted, aligned to a shared detection system, and multiplexed into a single reaction in just a couple of clicks.
Now get multiplex benefits without reoptimization
The UM Converter upgrades your SYBR assays so you’re able to:
- Get a multiplexed output
- Keep your primer target specificity
Avoid the manual legwork of primer design and probe planning
Ready to switch from singleplex SYBR to Universal Multiplexing? Find the UM converter in the Support Center.
