One-step RT-PCR. Zero compromises.
Transform your RT-PCR workflow
True RNA quantification
Direct molecular counting enables precise measurements with consistently low CVs
Superior sensitivity
Resolve alleles down to 0.004% MAF while maintaining linear performance across a wide dynamic range
Fewer steps, less error
One-step reverse transcription and amplification streamlines workflow and improves reproducibility
Seamless compatibility
Use existing probes, no assay redesign needed
Applications
Effortlessly upgrade your RT-dPCR or RT-qPCR workflows.
See how Countable RT-cPCR is transforming:
Workflow
A workflow simple enough to be adopted by any lab.
01. Prepare
Prepare reaction
Load RNA sample and reaction components to spin column.
02. Generate Matrix
Generate matrix
Centrifuge to isolate single molecules.
03. Amplify
Amplify targets
Perform PCR to amplify target signal.
04. Count
Count targets
Quantify using 3D light sheet imaging.
Key specifications
The performance is Countable.
30 in 1,000,000
Target molecules detected
6-log
Dynamic range
<1% CVs
High reproducibility
1- 96 samples
per run
1-35 µL
sample input
Up to 1 million
target molecules counted
Workflow
Getting started is simple.
01.
Design your assay
Getting started with a new or existing assay is easy. Choose the path that fits your workflow.
02.
Run your assay
Set up your reaction and detect targets using Countable reagents and instruments.
Countable Reagents
Countable Instruments
03.
Analyze your assay
The included software on the Countable instrument provides built-in tools for confident, compliant analysis.
Find out how Countable PCR transforms your work.
Frequently Asked Questions
Yes - we have Pre-designed Assays that include expert-verified protocols and performance data for commonly tested targets. We are continually adding new assays to our menu.
It is simple to transition existing hydrolysis probe-based assays to Countable PCR - your existing probes and primers can be used directly, although we do recommend including a probe additive to reduce background signal. Learn more here.
Yes. The software automatically detects and counts target molecules from the 3D light sheet images for every sample and target. In addition, every run produces a QC report in a 21 CFR Part 11–compliant format, supporting GMP-ready traceability.
It will depend on which Countable System is used for analysis. You can see which dyes are supported on the instrument pages for Countable 4 and Countable 10.