PCR without the complexity.
The new standard in PCR performance.
True quantification
Direct molecular counting enables precise measurements with consistently low CVs
Superior sensitivity
Resolve alleles down to 0.004% MAF while maintaining linear performance across a wide dynamic range
Effortless multiplexing
Rapidly develop multiplex assays, saving valuable time and resources
Seamless compatibility
Use existing probes, no assay redesign needed
Applications
See how Countable PCR redefines what’s possible across genomics applications.
Workflow
A workflow simple enough to be adopted by any lab.
01. Prepare
Prepare reaction
Load sample and reaction components to spin column.
02. Generate Matrix
Generate matrix
Centrifuge to isolate single molecules.
03. Amplify
Amplify targets
Perform PCR to amplify target signal.
04. Count
Count targets
Quantify using 3D light sheet imaging.
Compatible sample types
Cell free DNA
Tissue DNA
FFPE DNA
cDNA
Plasmids
Nucleosomal DNA
Genomic DNA, sheared
Genomic DNA, undigested
Crude cell lysate
Virus with capsids
Key specifications
The performance is Countable.
<1% CVs
High reproducibility
Up to 10 targets*
Multiplexing per reaction
1- 96 samples
per run
1-35 µL
sample input
up to 5kb
amplicon length
Up to 1 million
Target molecules counted
* Available on Countable 10. Contact us for service projects today.
Getting started is simple
Assay Design
Target amplification
Data Analysis
01. Assay design
Turn your ideas into results fast with better multiplexing and collaborative services.
Pre-designed Assays
Accelerate your research with pre-tested assays from the Countable Labs community.
Learn more
Assay services
Partner with Countable Labs for collaborative guidance to make the most of your assays.
Learn more
Universal Multiplexing
Develop and deploy multiplex assays in days, for 1/10th the cost of hydrolysis probes.
Learn more
02. TARGET AMPLIFICATION
Prepare 30 million compartments for bias-free amplification with no dead volume.
Countable Matrix Reagents Kit
Turn a 50 µL reaction into 30 million compartments with a simple centrifugation step. Supports 1–35 µL sample input.
Countable PCR Mix Kit
Get high-fidelity amplification for accurate, precise target quantification, for both Universal Multiplex and hydrolysis probes.
Countable Matrix Consumables Kit
Prepare up to 96 samples per run using standard PCR tubes. Flexible throughput handling with barcoded stripes for data traceability.
03. Data analysis
Support GMP workflows with software that gives you direct, objective counts and built-in QC metrics.
Measure directly in the reaction tube for loss-free quantification via 3D light sheet imaging.
Plus, you get integrated software and local analysis with built-in QC metrics and 21 CFR Part 11 support, delivering objective, audit-ready results.
Countable 10
Screen more targets than ever before — up to 10 colors — with clear, cross-talk free data.
Find out how Countable PCR transforms your work.
Frequently Asked Questions
Yes - we have Pre-designed Assays that include expert-verified protocols and performance data for commonly tested targets. We are continually adding new assays to our menu.
It is simple to transition existing hydrolysis probe-based assays to Countable PCR - your existing probes and primers can be used directly, although we do recommend including a probe additive to reduce background signal. Learn more here.
Yes. The software automatically detects and counts target molecules from the 3D light sheet images for every sample and target. In addition, every run produces a QC report in a 21 CFR Part 11–compliant format, supporting GMP-ready traceability.
It will depend on which Countable System is used for analysis. You can see which dyes are supported on the instrument pages for Countable 4 and Countable 10.