One-step RT-PCR. Zero compromises.
Transform your RT-PCR workflow
True RNA quantification
Direct molecular counting enables precise measurements with consistently low CVs
Superior sensitivity
Resolve alleles down to 0.004% MAF while maintaining linear performance across a wide dynamic range
Fewer steps, less error
One-step reverse transcription and amplification streamlines workflow and improves reproducibility
Seamless compatibility
Use existing probes, no assay redesign needed
Applications
Effortlessly upgrade your RT-dPCR or RT-qPCR workflows.
Workflow
A workflow simple enough to be adopted by any lab.
01. Prepare
Prepare reaction
Load RNA sample and reaction components to spin column.
02. Generate Matrix
Generate matrix
Centrifuge to isolate single molecules.
03. Amplify
Amplify targets
Perform PCR to amplify target signal.
04. Count
Count targets
Quantify using 3D light sheet imaging.
Key specifications
The performance is Countable.
30 in 1,000,000
Target molecules detected
6-log
Dynamic range
<1% CVs
High reproducibility
1- 96 samples
per run
1-35 µL
sample input
Up to 1 million
target molecules counted
Getting started is simple
Assay Design
Target amplification
Data Analysis
01. Assay design
Turn your ideas into results fast with better multiplexing and collaborative services.
Pre-designed Assays
Accelerate your research with pre-tested assays from the Countable Labs community.
Learn more
Assay services
Partner with Countable Labs for collaborative guidance to make the most of your assays.
Learn more
02. TARGET AMPLIFICATION
Prepare 30 million compartments for bias-free amplification with no dead volume.
Countable Matrix Reagents Kit
Turn a 50 µL reaction into 30 million compartments with a simple centrifugation step. Supports 1–35 µL sample input.
Countable PCR Mix Kit
Get high-fidelity amplification for accurate, precise target quantification, for both Universal Multiplex and hydrolysis probes.
Countable Matrix Consumables Kit
Prepare up to 96 samples per run using standard PCR tubes. Flexible throughput handling with barcoded stripes for data traceability.
RNA Module for Countable PCR
Directly reverse transcribe and amplify RNA in a single reaction for accurate, ultra-sensitive RNA quantification, for hydrolysis probes.
03. Data analysis
Support GMP workflows with software that gives you direct, objective counts and built-in QC metrics.
Measure directly in the reaction tube for loss-free quantification via 3D light sheet imaging.
Plus, you get integrated software and local analysis with built-in QC metrics and 21 CFR Part 11 support, delivering objective, audit-ready results.
Countable 10
Screen more targets than ever before — up to 10 colors — with clear, cross-talk free data.
Find out how Countable PCR transforms your work.
Frequently Asked Questions
Yes - we have Pre-designed Assays that include expert-verified protocols and performance data for commonly tested targets. We are continually adding new assays to our menu.
It is simple to transition existing hydrolysis probe-based assays to Countable PCR - your existing probes and primers can be used directly, although we do recommend including a probe additive to reduce background signal. Learn more here.
Yes. The software automatically detects and counts target molecules from the 3D light sheet images for every sample and target. In addition, every run produces a QC report in a 21 CFR Part 11–compliant format, supporting GMP-ready traceability.
It will depend on which Countable System is used for analysis. You can see which dyes are supported on the instrument pages for Countable 4 and Countable 10.