Consistent BCR::ABL1 quantification across four transcripts in one reaction

Summary

Consistent BCR::ABL1 monitoring requires quantification across a broad range of transcript levels. Conventional RT-qPCR workflows typically rely on external calibration curves and may require separate tests for baseline transcript identification and deep molecular monitoring.

The Ancora BCR::ABL1 Assay uses direct single-molecule counting across approximately 30 million compartments to identify and quantify four fusion transcripts, e14a2 and e13a2 p210, e1a2 p190, and e19a2 p230, alongside ABL1 in one closed reaction. This provides more complete transcript information from each sample without adding workflow complexity.

Download this application note to see how the Ancora BCR::ABL1 Assay performed across analytical, reference-panel, and clinical-sample studies, including:

• Single-reaction quantification of e14a2, e13a2, e1a2, e19a2, and ABL1, with separate resolution of both p210 transcripts
• Linear quantification from 10% to 0.0032% BCR::ABL1/ABL1 across all four fusion transcripts
• Calculated, input-dependent sensitivity of 0.007% at 100,000 ABL1 copies and 0.0007% at one million ABL1 copies
• Separate resolution of both p210 transcripts in dual-p210 samples and detection of p230 across longitudinal time points
• A closed 50 µL workflow with practically no dead volume, fixed-rule analysis, quality control, and automated reporting