Single-tube multiplex quantification of BCR::ABL1 fusion transcripts enables sensitive MRD monitoring across Isoforms in CML/ALL

Quantitative monitoring of BCR::ABL1 transcripts is central to MRD assessment in CML and B-ALL. Current clinical workflows rely on RT-qPCR or digital PCR (dPCR), which require multi-step processing and, in the case of qPCR, calibration to external standards for quantification. A simplified approach that enables direct, absolute quantification of BCR::ABL1 transcripts without reliance on standard curves could offer an alternative paradigm for MRD monitoring. This poster demonstrates how a single-molecule counting PCR approach enables enhanced clinical utility by simultaneously detecting p210, p190, and p230 transcript variants with high precision using a simplified workflow.