Multiplexed BCR::ABL1 panel on Countable RT-PCR enables ultrasensitive quantification of four BCR::ABL1 fusion variants in a single tube

The BCR::ABL1 fusion gene, produced by the Philadelphia chromosome translocation, is the genetic hallmark of chronic myeloid leukemia (CML), and accurately quantifying its transcripts while distinguishing clinically relevant isoforms is central to CML research, including MRD studies. Conventional RT-qPCR requires standard curves and extensive assay optimization, and its multiplexing capacity is limited when measuring multiple fusion transcripts and reference targets together. This poster presents a multiplex BCR::ABL1 RNA fusion panel on Countable PCR that uses a multi-probe labeling strategy to differentiate and quantify e14a2 and e13a2 (p210), e1a2 (p190), and e19a2 (p230) variants alongside the ABL1 reference gene in a single tube, with high sensitivity and specificity.