Development of a multiplexed NPM1 panel by single-molecule RNA counting for differential detection of Type A, B, and D mutations for AML MRD research applications

NPM1 mutations occur in about 30% of adult AML cases, with Types A, B, and D accounting for roughly 90%. Because they’re absent in healthy hematopoietic cells, these mutations are strong MRD research targets, and tracking a specific index mutation is key to distinguishing true clonal relapse from secondary or donor-derived events. Existing qPCR and fragment analysis methods require separate reactions per mutation type and calibration standards, while NGS adds cost, turnaround time, and bioinformatics complexity. This poster presents a multiplex NPM1 mutation panel on Countable PCR, a single-molecule counting platform that delivers type-specific detection and absolute quantification in one reaction for longitudinal research of AML disease burden