Rapid, quantitative viral genome integrity measurements using multiplex Countable PCR assays

Summary



This Application Note demonstrates the use of Countable Labs’ Universal Multiplex (UM) chemistry to design 3-plex integrity assays targeting various regions of the AAV genome, including the transgene. Additionally, the Countable System’s linkage module is used to measure AAV genome integrity via co-detection of the three regions on the vector genome.

Summary



This Application Note demonstrates the use of Countable Labs’ Universal Multiplex (UM) chemistry to design 3-plex integrity assays targeting various regions of the AAV genome, including the transgene. Additionally, the Countable System’s linkage module is used to measure AAV genome integrity via co-detection of the three regions on the vector genome.

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