Rapid, quantitative viral genome integrity measurements using multiplex Countable PCR assays
Summary
Genome integrity is a critical quality attribute in viral vector manufacturing — but current approaches have limited accuracy or require combining multiple readouts for quantification. Countable PCR takes a different approach: true single-molecule isolation across 30 million compartments enables direct DNA linkage analysis, so you can easily measure complete vs partial genomes with high accuracy in a single assay.
Download this application note to see how a 3-plex Countable PCR genome integrity assay performed on synthetic AAV genome proxies and a purified AAV sample, including:
- Single-molecule resolution with no co-occupancy artifacts or Poisson assumptions
- CVs under 3% across all complete:partial genome ratios tested
- Concordance with orthogonal methods — ~53% full-length genome content in a purified AAV sample, consistent with AUC and AEX
- Flexible assay design — Universal Multiplex chemistry enables incorporation of a gene of interest (GOI) with minimal optimization
