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Sensitive, reproducible mutation detection from cfDNA remains a critical challenge in oncology particularly when the variants that matter most are present at very low frequencies.

This joint webinar, presented in collaboration with Countable Labs and Promega, walks through a complete workflow from cfDNA extraction using the Promega Maxwell® System followed by ultra-sensitive variant detection using Countable PCR. Using multiplexed KRAS allele detection and BCR-ABL isoform analysis as real-world examples, attendees will learn how pairing quality extraction with single-molecule counting PCR delivers higher sensitivity and reproducibility than qPCR, dPCR, or NGS with a simpler workflow and lower cost per sample.

Key topics:

• Sensitivity where it counts: a complete cfDNA workflow for detecting clinically relevant variants down to 0.07% variant allele frequency (VAF) for KRAS G12C
• Reproducibility without the burden: how single-molecule counting PCR eliminates standard curves, Poisson statistics, and reduces replicates, giving labs consistent, confidence-worthy results run-to-run
• Lower cost per sample, simple operations: how automating extraction and consolidating multiple targets into a single tube, all relevant BCR-ABL isoforms in one reaction, or multiple KRAS variants cuts reagent usage and hands-on time without compromising sensitivity
• A complete cfDNA workflow from extraction to result: how pairing automated extraction with single-molecule counting PCR enables a faster and more streamlined high-confidence workflow for variant calls 

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A Q&A follows the presentation.